The Celtics capitalized on 22 turnovers by the Bulls, who dropped their second straight and third in four games.“They came out and hit us in the mouth in the second quarter,” Chicago coach Fred Hoiberg said. “A huge thing in this league is, find a way to fight through adversity and play through tough times, and we obviously did not do that well tonight.”Jabari Parker scored 14 in the first quarter for the Bulls, but was shut out the rest of the way.Shaquille Harrison led Chicago with 16 points. Zach LaVine had 10 points and nine assists, and Wendell Carter Jr. added 11 points and seven rebounds.“We just weren’t scoring the ball,” LaVine said. “We were playing really good defense, but the flood gates opened up and we couldn’t respond.”ADVERTISEMENT Nadine Lustre’s phone stolen in Brazil Boston Celtics guard Kyrie Irving (11) fakes out Chicago Bulls guard Ryan Arcidiacono (51) as he dribbles behind his back on a drive to the basket during the first quarter of an NBA basketball game in Boston, Wednesday, Nov. 14, 2018. (AP Photo/Charles Krupa)BOSTON — The Celtics returned from a dreadful road trip with a resounding rout.Kyrie Irving had 17 points and seven assists and Jaylen Brown scored 18 as Boston beat the Chicago Bulls 111-82 on Wednesday night.ADVERTISEMENT MOST READ Gasol, Grizzlies hold off Bucks late for 116-113 win Bulls: F Lauri Markkanen (right elbow sprain) is still a few weeks out from playing for the first time this season, Hoiberg said. “Lauri was able to increase his activity today and did not have any pain after, which is a great sign. Still same timeline — a couple weeks away,” Hoiberg explained.Celtics: F Marcus Morris sat out with an illness, missing his first game of the season. Morris came off the bench in the first 13 games and is fourth on the team in scoring at 11.8 points per game. “We’re better with Marcus. There’s no question about that. We want him back ASAP,” Stevens said.UP NEXTThe Bulls visit Milwaukee on Friday night.The Celtics host Toronto on Friday night.Sports Related Videospowered by AdSparcRead Next Don’t miss out on the latest news and information. Lights inside SMX hall flicker as Duterte rants vs Ayala, Pangilinan anew LATEST STORIES The Celtics dominated the second quarter, holding Chicago to 11 points on five field goals, and continued to pull away in the second half. They held a 22-point lead entering the fourth quarter and emptied their bench. The reserves stretched the advantage to 33 before it was over.Terry Rozier had 12 points for Boston, and Aaron Baynes finished with nine points and 11 rebounds.After going 9 for 23 in the first quarter, the Celtics outscored Chicago 32-11 in the second and forced the Bulls into seven turnovers.Rozier’s free throw after a technical foul on LaVine broke a 28-all tie and started Boston on a 12-0 run. The Celtics shut out the Bulls for 5:14 while pulling ahead 42-30, then closed the period on a 9-2 spurt for a 51-35 halftime lead.Baynes opened the fourth quarter for Boston with a 3-pointer and added a three-point play with 9:46 remaining to extend the margin to 87-64.Hayward followed with a jumper, then converted another three-point play as the Celtics opened a 92-64 bulge with 9:32 left.TIGHT DThe Celtics made a season-high 12 steals, led by Hayward’s three, and blocked five shots. Chicago’s 82 points marked the lowest total allowed by Boston this season.“We were prepared. I think that we’ve been prepared other games but we’ve got to match that with the attitude and effort to execute on both ends,” Irving said.TIP-INS Gretchen Barretto’s daughter Dominique graduates magna cum laude from California college Japeth Aguilar embraces role, gets rewarded with Finals MVP plum Gov’t to employ 6,000 displaced by Taal View comments Allen Durham still determined to help Meralco win 1st PBA title Lights inside SMX hall flicker as Duterte rants vs Ayala, Pangilinan anew “One of the toughest things to do in this league is to come off a road trip, good or bad, and play well in front of the home fans,” Irving said. “For us it was a conscious effort to be more aggressive and kind of play the game out as it came.”The Celtics were back home for the first time since going 1-4 on a long trip that wrapped up with back-to-back losses in Utah and Portland. After a slow start through the first quarter, Boston started pulling away from the Bulls in the second quarter and never let up.FEATURED STORIESSPORTSGinebra beats Meralco again to capture PBA Governors’ Cup titleSPORTSJapeth Aguilar wins 1st PBA Finals MVP award for GinebraSPORTSGolden State Warriors sign Lee to multiyear contract, bring back Chriss“Once we hit a couple shots, I thought we felt a lot better about ourselves,” coach Brad Stevens said.Gordon Hayward scored 11 and Al Horford had 10 points and eight rebounds as all five Boston starters reached double figures. Will you be the first P16 Billion Powerball jackpot winner from the Philippines? Carpio hits red carpet treatment for China Coast Guard PLAY LIST 02:14Carpio hits red carpet treatment for China Coast Guard02:56NCRPO pledges to donate P3.5 million to victims of Taal eruption00:56Heavy rain brings some relief in Australia02:37Calm moments allow Taal folks some respite03:23Negosyo sa Tagaytay City, bagsak sa pag-aalboroto ng Bulkang Taal01:13Christian Standhardinger wins PBA Best Player award Tim Cone, Ginebra set their sights on elusive All-Filipino crown
During a campaign stop in Dubuque, Iowa, on Thursday, Giuliani was asked whether he still stands by Kerik. He sidestepped that question and said the issue has to be decided by the courts. “A lot of public comment about it is inconsistent with its getting resolved in the right way in the courts,” Giuliani said. A former undercover police officer with a bodybuilder’s physique and a knack for charming people in high places, Kerik has since been hit with a string of revelations about personal and professional improprieties. His nomination was confronted with news reports about stock-option windfalls, his connections with people suspected of doing business with the mob and overlapping extramarital affairs with two women: Judith Regan, the publisher of his memoir, and a city correction officer. The liaisons reportedly occurred in an apartment near ground zero that had been set aside for rescue workers. Kerik, 51, who married his current wife in 1998 and has two children with her, apparently became close with Regan while writing “The Lost Son,” in which he described being abandoned by his prostitute mother. Kerik rose from cop to Giuliani’s correction commissioner in the late 1990s. From there, he became police commissioner and later went to work in Iraq rebuilding the country’s police force. Then came the failed Homeland Security nomination. Democrats who opposed the nomination focused on Kerik’s recent windfall from exercising stock options in a stun-gun company that did business with the department. His take: $6.2 million. Days after President George W. Bush introduced Kerik as his nominee, Kerik announced he was withdrawing his name because of tax issues involving his former nanny. But by then, state investigators were already aware of the expensive renovations done to his Bronx apartment in 1999, including built-in cabinets and a rotunda with a marble entryway. They alleged the work was paid for by Mafia-connected builders who sought his help winning city contracts. Giuliani was forced to testify before a state grand jury in a case that resulted in Kerik pleading guilty last year to accepting illegal gifts while on the city payroll.160Want local news?Sign up for the Localist and stay informed Something went wrong. Please try again.subscribeCongratulations! You’re all set! AD Quality Auto 360p 720p 1080p Top articles1/5READ MOREStriving toward a more perfect me: Doug McIntyre Several calls to Kerik’s lawyer, Kenneth Breen, were not immediately returned. Authorities have alleged that Kerik took tens of thousands of dollars in services from benefactors and never reported it as income. Earlier this year, he rejected a plea deal, and his attorney insisted he had done nothing wrong. An indictment is the latest chapter of a downfall that began within days of Kerik’s nomination in 2004 to head the Department of Homeland Security. At the time, he was billed by the former mayor as a no-nonsense, self-made lawman who helped restore calm following the Sept. 11, 2001, terrorist attacks. A federal indictment of Kerik could complicate matters for Giuliani as the first presidential primaries draw near. The ex-mayor frequently says that he made a mistake in recommending Kerik to be Homeland Security chief, but that might not be enough to avoid the political damage of a drawn-out criminal case involving his one-time protege. NEW YORK – A federal grand jury has indicted Bernard Kerik, the former New York City police commissioner under former Mayor Rudy Giuliani, on corruption charges, a person close to the investigation said Thursday. The charges include mail and wire fraud, tax fraud, making false statements on a bank application, making false statements for a U.S. government position and theft of honest services, the person said. The theft charge essentially accuses a government employee of abusing his position and defrauding the public. The person spoke on condition of anonymity because the indictment was sealed and wasn’t expected to be unsealed until Kerik’s arraignment today.
Welcome to The Lab, FiveThirtyEight’s basketball podcast. On this week’s show (Nov. 8, 2017), Neil and Kyle break down the news that the Phoenix Suns have agreed to trade Eric Bledsoe to the Milwaukee Bucks. We also get into what exactly is going on with the Cleveland Cavaliers — and just how their latest woes may affect their playoff chances. Next, Baxter Holmes joins for a discussion of ESPN’s “schedule alert” project, which identifies games that NBA teams are likely to lose because of the punishing 82-game schedule. Plus, a significant digit on the Detroit Pistons.Here are links to what we discussed this week:LeBron can’t do it all alone, writes Kyle.Baxter Holmes’s schedule alert project.Neil investigates the Detroit Pistons’ new, improved habits on offense. FiveThirtyEight Embed Code More: Apple Podcasts | ESPN App | RSS | Embed
All-you-can-eat charcuterie and cheese at Venissimo Cheese Del Mar KUSI Newsroom, February 15, 2018 Posted: February 15, 2018 KUSI Newsroom Categories: Local San Diego News FacebookTwitter 00:00 00:00 spaceplay / pause qunload | stop ffullscreenshift + ←→slower / faster ↑↓volume mmute ←→seek . seek to previous 12… 6 seek to 10%, 20% … 60% XColor SettingsAaAaAaAaTextBackgroundOpacity SettingsTextOpaqueSemi-TransparentBackgroundSemi-TransparentOpaqueTransparentFont SettingsSize||TypeSerif MonospaceSerifSans Serif MonospaceSans SerifCasualCursiveSmallCapsResetSave SettingsFree wine and all-you-can-eat cheese and charcuterie and it’s all part of Venissimo Cheese Happy Hour Extravaganza.KUSI’s Ginger Jeffries was LIVE with all the details.
WILMINGTON, MA — Below is advice from the Wilmington Board of Health published in the most recently Town Topics newsletter:Mosquito and tick season is upon us, the Board of Health would like to remind residents to take precautions to reduce their risk of exposure to mosquitoes and ticks.MOSQUITOES – Try to avoid outdoor activity during times of day with increased mosquito activity, which is dawn and dusk. Use mosquito repellents containing an EPA-approved active ingredient such as DEET.Residents should wear long sleeved clothing to reduce mosquito access to skin and remove any standing water in your yard, including children’s toys, birdbaths, pots and planters.Wilmington is part of the Central Massachusetts Mosquito Control Project and routine spraying and larvicide treatment is conducted throughout the summer.TICKS – Ticks are prevalent in Wilmington. Ticks like damp, shady, brushy, leafy areas. If you have been in an area where ticks might be present, it is important to check yourself, your family and your pets for ticks every day. Use repellents that contain DEET on your exposed skin and those that contain permethrin on your clothes. When spending time outside, if weather permits, wear long-sleeved, light colored shirts and long pants tucked into your socks.Keep the grass cut short and remove piles of leaves and brush. Talk to your veterinarian about the best ways to protect your pets and livestock from ticks.If you find a tick on yourself, remove it carefully as soon as possible. If you develop a rash or other signs of illness following a tick bite, be sure to contact your health care provider immediately. If you are looking into preventative tick control, check out tick tubes, which may be found online. They work great ridding areas of deer ticks only. Remember deer ticks are the ones that transmit Lyme Disease. If you have been bitten by a tick, log on to the Board of Health website for links to get the ticks tested.Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email email@example.com.Share this:TwitterFacebookLike this:Like Loading… RelatedWilmington Board Of Health Offers Advice On Mosquitoes & TicketsIn “Government”Wilmington Board Of Health Urges Residents To Use Caution As Mosquitos Test Positive For EEE In AndoverIn “Government”Request A Free Mosquito Spraying From Central Mass. Mosquito Control Project This SeptemberIn “Government”
BNP senior leader Moudud Ahmed. Prothom Alo File PhotoBNP standing committee member Moudud Ahmed alleged that policemen barred him from leaving his house in Manikpur village of Companiganj upazila in Noakhali district to exchange greeting with supporters on the Eid day, Saturday.Police, however, denied the allegation citing ‘the blockade was for security reasons’, reports UNB.The Bangladesh Nationalist Party standing committee member Moudud told newsmen that it has been proved that this government has become isolated from people and also being feared to face mass people.”So, policemen were deployed in front of my house in the morning and they barred me from coming out of my home,” he added.”I asked police to arrest me if I did anything wrong or let me go out, but they were firm about not allowing me to go outside,” said the senior BNP leader.Moudud said he spoke to Companiganj police station officer-in-charge and superintendent of police in Noakhali Md Ilias Sharif. Both denied him permission to go out, he added.When contacted, Md Ilias Sharif said, “Moudud Ahmed is a senior political leader. We deployed police in front of his house considering his security.”Rabiul Islam, sub-inspector (investigation) of Companiganj police station, who was on duty in front of Moudud’s house, said police were deployed following an intelligence report that he might face problems if he went out of home just before evening.
Randi Weingarten tries to deliver a teddy bear and other items for children to federal agents at the port-of-entry, Tuesday on 26 June 2018, in Fabens, Texas, along the international border where immigrant children are being held. The group tried to deliver items to the children housed in tents at the facility but were turned away. Photo: APA judge in California on Tuesday ordered US border authorities to reunite separated families within 30 days, setting a hard deadline in a process that has so far yielded uncertainty about when children might again see their parents.If children are younger than 5, they must be reunified within 14 days of the order issued Tuesday by US district judge Dana Sabraw in San Diego. Sabraw, an appointee of President George W. Bush, also issued a nationwide injunction on future family separations, unless the parent is deemed unfit or doesn’t want to be with the child. He also requires the government provide phone contact between parents and their children within 10 days.More than 2,000 children have been separated from their parents in recent weeks and placed in government-contracted shelters – hundreds of miles away, in some cases – under a now-abandoned policy toward families caught illegally entering the USAmid an international outcry, Trump last week issued an executive order to stop the separation of families and said parents and children will instead be detained together. A Department of Homeland Security statement over the weekend on reuniting families only seemed to sow more confusion.”The facts set forth before the Court portray reactive governance_responses to address a chaotic circumstance of the Government’s own making,” Sabraw wrote. “They belie measured and ordered governance, which is central to the concept of due process enshrined in our Constitution.”The ruling was a win for the American Civil Liberties Union, which filed the lawsuit in March involving a 7-year-old girl who was separated from her Congolese mother and a 14-year-old boy who was separated from his Brazilian mother.”Tears will be flowing in detention centers across the country when the families learn they will be reunited,” said ACLU attorney Lee Gelernt.The Justice and Homeland Security Departments did not immediately respond to requests for comment late Tuesday.It’s not clear how border authorities will meet the deadline. Health and Human Services Secretary Alex Azar told Congress on Tuesday that his department still has custody of 2,047 immigrant children separated from their parents at the border. That is only six fewer children than the number in HHS custody as of last Wednesday. Democratic senators said that wasn’t nearly enough progress.Under questioning, Azar refused to be pinned down on how long it will take to reunite families. He said his department does extensive vetting of parents to make sure they are not traffickers masquerading as parents.Also challenging will be the requirement the judge set on phone contact.At a Texas detention facility, immigrant advocates complained that parents have gotten busy signals or no answers from a 1-800 number provided by federal authorities to get information about their children.Attorneys have spoken to about 200 immigrants at the Port Isabel detention facility near Los Fresnos, Texas, since last week, and only a few knew where their children were being held, said Simon Sandoval-Moshenberg of the Legal Aid Justice Center in Virginia.”The US government never had any plan to reunite these families that were separated,” Sandoval-Moshenberg said, and now it is “scrambling to undo this terrible thing that they have done.”A message left for HHS, which runs the hotline, was not immediately returned.Many children in shelters in southern Texas have not had contact with their parents, though some have reported being allowed to speak with them in recent days, said Meghan Johnson Perez, director of the Children’s Project for the South Texas Pro Bono Asylum Representation Project, which provides free legal services to minors.”Things might be changing now. The agencies are trying to coordinate better,” she said. “But the kids we have been seeing have not been in contact with the parents. They don’t know where the parent is. They’re just distraught. Their urgent need is just trying to figure out, ‘Where is my parent?'”The decision comes as 17 states, including New York and California, sued the Trump administration Tuesday to force it to reunite children and parents. The states, all led by Democratic attorneys general, joined Washington, DC, in filing the lawsuit in federal court in Seattle, arguing that they are being forced to shoulder increased child welfare, education and social services costs. The Justice Department did not immediately respond to a request for a comment on the multistate lawsuit.”The administration’s practice of separating families is cruel, plain and simple,” New Jersey Attorney General Gurbir Grewal said in a statement. “Every day, it seems like the administration is issuing new, contradictory policies and relying on new, contradictory justifications. But we can’t forget: The lives of real people hang in the balance.”In a speech before the conservative Criminal Justice Legal Foundation in Los Angeles, U.S. Attorney General Jeff Sessions defended the administration for taking a hardline stand on illegal immigration and said the voters elected President Donald Trump to do just that.”This is the Trump era,” he said. “We are enforcing our laws again. We know whose side we are on – so does this group – and we’re on the side of police, and we’re on the side of the public safety of the American people.”After expressing reluctance in May to get too deeply involved in immigration enforcement decisions, the judge who issued Tuesday’s ruling was clearly influenced by Trump’s reversal last week and the Homeland Security Department’s statement on its family reunification plan Saturday night, which, he said, left many questions unanswered.”This situation has reached a crisis level. The news media is saturated with stories of immigrant families being separated at the border. People are protesting. Elected officials are weighing in. Congress is threatening action,” he wrote.Outraged by the family separations, immigrant supporters have led protests in recent days in states such as Florida and Texas. In Los Angeles, police arrested 25 demonstrators at rally Tuesday ahead of Sessions’ address.Outside the U.S. attorney’s office, protesters carried signs reading, “Free the children!” and “Stop caging families.” Clergy members blocked the street by forming a human chain. Police handcuffed them and led them away.Later, protesters gathered outside the hotel where Sessions gave his speech. As the attorney general’s motorcade arrived, the crowd chanted, “Nazi, go home.”Weissert reported from Harlingen, Texas. Associated Press writers Ricardo Alonso-Zaldivar in Washington; Gene Johnson in Seattle; Robin McDowell in Austin, Texas; Amy Taxin in Santa Ana, California; and John Antczak in Los Angeles contributed to this report.
Share 00:00 /01:08 – / 3Woodlands Express is a commuter bus service that’s operated by The Woodlands Township, with the help of a federal transit grant. About 2,700 riders a day use the service to get from The Woodlands to downtown Houston. It also stops in the Med Center and Greenway Plaza. But when the buses head back to Montgomery County, they’re empty. To fill those seats, The Woodlands Township Board of Directors has approved a new reverse-commuting program. The Woodlands Director of Community Relations Nick Wolda says the buses will now start carrying people from Houston to the suburbs.“There’s really no risk on our part as far as the financial risk because these are buses already coming back,” says Wolda. But once you get riders to The Woodlands, how do you get them to the places where they work? Wolda says they plan to discuss that with some of the area’s major employers. “Those are things we’ll be working through over the next few weeks,” adds Wolda. “And we plan to launch this service once we develop and work out a lot of these details.” Woodlands Express is planning to start the reverse commuting program after the first of the year. The fare for a round-trip is currently $13. Listen X To embed this piece of audio in your site, please use this code:
The proposed model of cytosolic mobility: (Red box) objects (red spheres) larger than the pore size of the cytoskeleton (50-75 nm) are trapped in a viscoelastic medium and experience restricted diffusion, (green box) if objects are perfectly inert they diffuse freely without hindrance due to obstacles, (purple box) when objects interact with intracellular components diffusion is slowed down but remains Brownian, (yellow box) if interactions increase to the point that immobilization time is longer than the time of observation, the diffusion is significantly slowed down to become anomalous, known as subdiffusion. Credit: Nature Materials (2018) doi: 10.1038/s41563-018-0120-7 Play Representative videos of 25 nm fluorescently labelled NPs diffusing in HeLa cells after pinocytic loading for single-particle tracking experiments. Top panel: unburst vesicles with encapsulated NPs; particles in the intact vesicles move very fast and cannot be tracked individually. Bottom panel: examples of typical regions of interest selected for single-particle tracking analysis of NPs, systematically excluding the unburst vesicles. Behavioral difference of free versus encapsulated NPs ensured a reliable discrimination between the two cases. Images were acquired every 10 ms; video was encoded at 30 frames per second (fps). Scale bar 1 µm. Credit: Nature Materials (2018), doi: 10.1038/s41563-018-0120-7. A brief osmotic shock enabled the dispersion of NPs into the cytoplasm. The NP’s diffusion-based exploration of the cell’s interior was monitored by extensive single-particle tracking experiments. Using this approach, a variety of NP surface modifications and particle sizes ranging from 15 to 75 nm were observed to interact with the surroundings for pronounced diffusion anomalies. , Cell © 2018 Phys.org The experimental data were also explained with a minimal model based on continuous-time random walks, where particles were described to take power-law-distributed rests between periods of free diffusion motion. With this approach, the findings on MSD could be reproduced and captured as particle trajectories. Future work is required to explore if these results also apply to crowds of one to 10 nm-sized macromolecules in cells. Gaining an understanding of how anomalous random walks can be altered when modifying non-equilibrium conditions in cells (such as changed metabolic states) will keep both experimentalists and theorists busy. Overall, the researchers proposed a unified framework to describe the dynamics of diffusion related to nanosized objects in the cell cytoplasm. The study also provided a quantitative benchmarking tool to design perfectly inert NPs that could diffuse within cells unhindered by obstacles, and defined them as “lone maze runners.” Such insights may become crucial to design intracellular drug delivery pathways to easily reach molecular targets within cells in the future. Citation: Maze runners and square dancers: Cytosolic diffusion of nanosized objects in mammalian cells (2018, August 15) retrieved 18 August 2019 from https://phys.org/news/2018-08-maze-runners-square-dancers-cytosolic.html Cells are complex, multi-compartmentalized entities of matter enclosed with a variety of membrane-bound organelles ranging from the microscale (µm) down to the nanoscale (nm) in diameter. These structures intermingle in a crowded aqueous phase known as the cytoplasm, within which diffusion deviates from Brownian motion. Understanding the concept of “cell crowding” and the impact on intracellular mobility can enable controlled diffusion within cells for improved drug delivery and other medical applications. The eukaryotic cytoplasm is a biphasic poroelastic (fluid and solid interaction) medium, containing a fluid phase (cytosol with water and soluble proteins) and a solid phase (cytoskeleton and other organelles). PausePlay% buffered00:0000:00UnmuteMuteDisable captionsEnable captionsSettingsCaptionsDisabledQuality0SpeedNormalCaptionsGo back to previous menuQualityGo back to previous menuSpeedGo back to previous menu0.5×0.75×Normal1.25×1.5×1.75×2×Exit fullscreenEnter fullscreen Explore further , Reports on Progress in Physics More information: Fred Etoc et al. Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells, Nature Materials (2018). DOI: 10.1038/s41563-018-0120-7 Matthias Weiss. A tale about square dancers and maze runners, Nature Materials (2018). DOI: 10.1038/s41563-018-0126-1A. Einstein. Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen, Annalen der Physik (2007). DOI: 10.1002/andp.19053220806 Alice B. Fulton. How crowded is the cytoplasm?, Cell (2004). DOI: 10.1016/0092-8674(82)90231-8 Felix Höfling et al. Anomalous transport in the crowded world of biological cells, Reports on Progress in Physics (2013). DOI: 10.1088/0034-4885/76/4/046602 O. Bénichou et al. Geometry-controlled kinetics, Nature Chemistry (2010). DOI: 10.1038/nchem.622 Experiments conducted with tracers on intracellular motion have typically revealed both Brownian and subdiffusive motion (or anomalous diffusion). High concentrations of macromolecules in the cytoplasm described as “obstructive molecular crowding” and biochemical interactions (electrostatic or hydrophobic) naturally affect the mobility of intracellular tracers. As a result, a fundamental question has remained unresolved: Can a unified framework account for the large variability of behaviors observed in cytosolic diffusion? Now writing in Nature Materials, Fred Etoc and colleagues have used single-particle tracking of fluorescent nanoparticle (NP) tracers to observe and propose a unified framework that describes the diffusion dynamics of nanosized objects in the eukaryotic cell cytoplasm.The researchers demonstrate:Inert objects were unaffected by obstructive molecular crowding to show Brownian motion, diffusing in a medium of low viscosity—if they were below approximately 75 nm in size. The researchers metaphorically compared them to “maze runners”Tracers that were approximately 25 nm in size but with increased non-specific interactions slowed down diffusion by three orders of magnitude, to gradually become anomalous and were compared to “square dancers”The strength of non-specific interactions is captured into a single parameter using a simple continuous time random walk (CTRW) model, a textbook model that describes the random walk performed by diffusing particles. By fixing a few basic properties of the random walk, it was possible to observe that diffusing particles explored an area (mean square displacement – MSD) that grew linearly in time. To define the phenomenon of intracellular motion experimentally, Etoc and co-workers hijacked a cellular entry gate known as the pinocytosis pathway (observed with vesicle formation to transport biological materials), to import functionalized and fluorescently labelled nanoparticles (NPs) in to living HeLa cells. Cytoplasmic diffusion of individual NPs (20 to 50 NPs per cell) was recorded by time-lapse microscopy and quantified using single-particle tracking analysis. In the cytoplasm of living HeLa cells non-specific interactions mainly contribute to anomalous diffusion. By changing the surface properties of NPs from strong to almost vanishing interactions within the interior of living cells, the trajectories of interacting NPs (square dancers) were shown to exhibit significant anomalous diffusion (indicated by red regions in density plots). In contrast, non-interacting NPs (lonesome maze runners) underwent normal diffusion. Adapted from doi:10.1038/s41563-018-0120-7. Credit: Nature Materials (2018), doi: 10.1038/s41563-018-0126-1. In the study, Etoc et al. estimated mobility at short timescales by computing time averaged MSD at 60 ms, expressed in terms of the diffusion coefficient D60ms To represent the behavior of the whole population of NPs in each condition, the distribution of the parameters α (anomalous exponent) and D60ms arising from each individual trajectory, was presented as a two-dimensional (2-D) density map. Such density plots for hundreds of trajectories and conditions that integrated the results of α and D60ms were summarized elegantly in the findings. , Nature Chemistry The hidden order in DNA diffusion Journal information: Nature Materials This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.